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Introduction
Dissolved oxygen (DO) is an essential measurement parameter in aerobic bioreactors. The growth of all cells is heavily dependent on DO because it acts as a terminal electron acceptor in aerobic respiration. However, if excessive amount of DO is added to the process, it may limit the growth of the culture and promote undesirable organisms. Consequently, the measurement of DO is critical to effective operation of systems. Today, a variety of sensors are available in the market, each with its own advantages and disadvantages.
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The oxygen concentration is determined with the Stern-Volmer equation, that sets the relationship between luminescence lifetime (intensity) and oxygen concentration see Figure 4.
Figure 4. Stern-Volmer equation. Source: https://www.fondriest.com/pdf/ysi_do_handbook.pdf
The most significant advantage of an optical dissolved oxygen sensor is low maintenance cost and the possibility of less frequent calibration. Other advantages and disadvantages can be found from Figure 5.
Figure 5. Advantages and disadvantages of optical sensors. Source: https://www.fondriest.com/pdf/ysi_do_handbook.pdf
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- Since the electrode consumes oxygen, readings are affected by flow across the sensor tip. Thus enough flow rate at the membrane (or sample renewal rate) must be ensured for accurate results.
Galvanic Electrode
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Measuring dissolved oxygen with either sensor type
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