Study of Nuclear magnetic resonance by Qaiser Siddique, Vallo Zinin and Razib Hossain.
Basics:
Nuclear magnetic resonance builds on the physics concepts of resonance and nuclear spin(angular momentum of elementary particles of an atom). Protons, electrons, atomic nuclei, and other elementary particles have an intrinsic spin as if they were spinning on their axes.When atomic nuclei, or other charged particles, spin they become like a magnetic dipole (like a bar magnet).A nucleus, or elementary particle, possessing spin will wobble or precess when they are placed in a magnetic field. A nucleus in a magnetic field precesses at a frequency, called the Larmor frequency, which is proportional to the magnetic field.
When an atom is in a constant magnetic field, it nucleus precesses at the Larmor frequency. If in addition to the constant magnetic field, there is also a weaker perpendicular magnetic field that varies at the same frequency as the Larmor frequency for the nucleus, then the nucleus resonates. The phenomenon is called Nuclear Magnetic Resonance (NMR).
Analogy:
This nuclear magnetic resonance (NMR) causes the spin of the nucleus to flip.The analogy for Earth spinning on its axis would be if Earth flipped so that the north and south poles interchanged and Earth were suddenly spinning in the opposite direction. Just as it would take considerable energy to flip Earth's spinning motion, the nucleus absorbs and emits energy as it flips its spin. With the correct electronic equipment physicists can measure the spectrum of absorbed and emitted energy.
APPLICATIONS OF NMR
Solution structure The only method for atomic-resolution structure determination of biomacromolecules in aqueous solutions under near physiological conditions or membrane mimeric environments.
Molecular dynamics The most powerful technique for quantifying motional properties of biomacromolecules.
Protein folding The most powerful tool for determining the residual structures of unfolded proteins and the structures of folding intermediates.
Ionization state The most powerful tool for determining the chemical properties of functional groups in biomacromolecules, such as the ionization states of ionizable groups at the active sites of enzymes.
Weak intermolecular interactions Allowing weak functional interactions between macrobiomolecules (e.g., those with dissociation constants in the micromolar to millimolar range) to be studied, which is not possible with other technologies.
Protein hydration A power tool for the detection of interior water and its interaction with biomacromolecules.
Hydrogen bonding A unique technique for the DIRECT detection of hydrogen bonding interactions.
Drug screening and design Particularly useful for identifying drug leads and determining the conformations of the compounds bound to enzymes, receptors, and other proteins.
Native membrane protein Solid state NMR has the potential for determining atomic-resolution structures of domains of membrane proteins in their native membrane environments, including those with bound ligands.
Metabolite analysis A very powerful technology for metabolite analysis.
Chemical analysis A matured technique for chemical identification and conformational analysis of chemicals whether synthetic or natural.
Material science A powerful tool in the research of polymer chemistry and physics.
NMR Spectroscopy
One common application of nuclear magnetic resonance is NMR spectroscopy. Physicists and chemists study the NMR spectrum produced by a sample of material and deduce the properties of the nuclei in the sample. This tells them what elements are in the sample.
MRI Medical Imaging
A better known application of nuclear magnetic resonance is magnetic resonance imaging (MRI). MRI uses the nuclear magnetic resonance effect of the hydrogen atoms in the human body. Computer analysis of the resonance data produces an internal image of the patients body. MRI produces diagnostic medical imaging that neither harms the body in any way nor requires surgeons to cut open or enter the body.
Nuclear magnetic resonance is an interesting phenomenon taking place in the nuclei of atoms that also has very important applications.
Spectroscopy
Spectroscopy is the study of the interaction of electromagnetic radiation with matter. Nuclear magnetic resonance spectroscopy is the use of the NMR phenomenon to study physical, chemical, and biological properties of matter. As a consequence, NMR spectroscopy finds applications in several areas of science. NMR spectroscopy is routinely used by chemists to study chemical structure using simple one-dimensional techniques. Two-dimensional techniques are used to determine the structure of more complicated molecules. These techniques are replacing x-ray crystallography for the determination of protein structure. Time domain NMR spectroscopic techniques are used to probe molecular dynamics in solutions. Solid state NMR spectroscopy is used to determine the molecular structure of solids. Other scientists have developed NMR methods of measuring diffusion coefficients.
The versatility of NMR makes it pervasive in the sciences. Scientists and students are discovering that knowledge of the science and technology of NMR is essential for applying, as well as developing, new applications for it. Unfortunately many of the dynamic concepts of NMR spectroscopy are difficult for the novice to understand when static diagrams in hard copy texts are used. The chapters in this hypertext book on NMR are designed in such a way to incorporate both static and dynamic figures with hypertext. This book presents a comprehensive picture of the basic principles necessary to begin using NMR spectroscopy, and it will provide you with an understanding of the principles of NMR from the microscopic, macroscopic, and system perspectives.
MRI Basics
MRI machines make use of the fact that body tissue contains lots of water, and hence protons (1H nuclei), which get aligned in a large magnetic field.[4] Each water molecule has two hydrogen nuclei or protons. When a person is inside the powerful magnetic field of the scanner, the average magnetic moment of many protons becomes aligned with the direction of the field. A radio frequency current is briefly turned on, producing a varying electromagnetic field. This electromagnetic field has just the right frequency, known as the resonance frequency, to be absorbed and flip the spin of the protons in the magnetic field. After the electromagnetic field is turned off, the spins of the protons return to thermodynamic equilibrium and the bulk magnetization becomes re-aligned with the static magnetic field. During this relaxation, a radio frequency signal (electromagnetic radiation in the RF range) is generated, which can be measured with receiver coils.
Magnetic resonance spectroscopy (MRS) is used to measure the levels of different metabolites in body tissues. The MR signal produces a spectrum of resonances that correspond to different molecular arrangements of the isotope being "excited". This signature is used to diagnose certain metabolic disorders, especially those affecting the brain,[48] and to provide information on tumor metabolism.[49]
Magnetic resonance spectroscopic imaging (MRSI) combines both spectroscopic and imaging methods to produce spatially localized spectra from within the sample or patient. The spatial resolution is much lower (limited by the available SNR), but the spectra in each voxel contains information about many metabolites. Because the available signal is used to encode spatial and spectral information, MRSI requires high SNR achievable only at higher field strengths (3 T and above).
Magnetic Resonance Imaging
Proton nuclear magnetic resonance (NMR) detects the presence of hydrogens (protons) by subjecting them to a large magnetic field to partially polarize the nuclear spins, then exciting the spins with properly tuned radio frequency (RF) radiation, and then detecting weak radio frequency radiation from them as they "relax" from this magnetic interaction. The frequency of this proton "signal" is proportional to the magnetic field to which they are subjected during this relaxation process. In the medical application known as Magnetic Resonance Imaging (MRI), an image of a cross-section of tissue can be made by producing a well-calibrated magnetic field gradient across the tissue so that a certain value of magnetic field can be associated with a given location in the tissue. Since the proton signal frequency is proportional to that magnetic field, a given proton signal frequency can be assigned to a location in the tissue. This provides the information to map the tissue in terms of the protons present there. Since the proton density varies with the type of tissue, a certain amount of contrast is achieved to image the organs and other tissue variations in the subject tissue. 
Since the MRI uses proton NMR, it images the concentration of protons. Many of those protons are the protons in water, so MRI is particularly well suited for the imaging of soft tissue, like the brain, eyes, and other soft tissue structures in the head as shown at left. The bone of the skull doesn't have many protons, so it shows up dark. Also the sinus cavities image as a dark region.
Bushong's assessment is that about 80% of the body's atoms are hydrogen atoms, so most parts of the body have an abundance of sources for the hydrogen NMR signals which make up the magnetic resonance image.
The schematic below may help visualize the imaging process. It is presumed that there are two regions of the sample which contain enough hydrogens to produce a strong NMR signal. The top sketch visualizes an NMR process with a constant magnetic field applied to the entire sample. The hydrogen spin-flip frequency is then the same for all parts of the sample. Once excited by the RF signal, the hydrogens will tend to return to their lower state in a process called "relaxation" and will re-emit RF radiation at their Larmor frequency. This signal is detected as a function of time, and then is converted to signal strength as a function of frequency by means of aFourier transformation. Since the protons in each of the active areas of the sample are subjected to the same magnetic field, they will produce the same frequency of radiation and the Fourier transform of the detected signal will have only one peak. This one peak demonstrates the presence of hydrogen atoms, but gives no information to locate them in the sample.
Information about the location of the hydrogen atoms can be obtained by adding a calibrated gradient field across the region of the sample as shown in the bottom sketch above. With an increasing magnetic field as you move to the right across the sample, the spin-flip energy and therefore the frequency of the emitted signal increases from left to right. When excited by an RF transmitter, the emitted signal contains different frequencies for the two proton concentration areas. These frequencies can be separated by means of the Fourier transform and the example gives two different regions of frequency for the two sample areas. This is the beginning of the process of locating the hydrogen atoms. In the sketch, it only locates them along the horizontal direction, giving no indication that they are at different heights. 